Simultaneous Determination of Salicylic Acid and Salicyluric Acid in Plasma and Urine by High Pressure Liquid Chromatography
- 1 January 1977
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 10 (7-8) , 539-550
- https://doi.org/10.1080/00032717708059220
Abstract
A sensitive, rapid, and specific high pressure liquid chromatographic (HPLC) assay was developed for the determination of salicylic (SA) and salicyluric (SU) acids in plasma and urine. The compounds are extracted into ethyl ether at acid pH, evaporated, and reconstituted prior to instrumental separation. Overall recovery of both compounds is 90 ± 5%, and the sensitivity limits are 150 ng of SU and 300 ng SA per ml of biological fluid. The assay was used for the determination of both compounds in plasma and urine of man following oral doses of 40 mg/kg of sodium salicylate.Keywords
This publication has 3 references indexed in Scilit:
- Determination of acetylsalicylic acid and salicylic acid in plasmaJournal of Pharmaceutical Sciences, 1967
- Pharmacokinetics of Salicylate Elimination in ManJournal of Pharmaceutical Sciences, 1965
- Salicylate and Salicyl Conjugates: Fluorimetric Estimation, Biosynthesis and Renal Excretion in Man1Journal of Clinical Investigation, 1958