A Genetic Linkage Map of the Apicomplexan Protozoan Parasite Eimeria tenella
Open Access
- 1 October 2000
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 10 (10) , 1587-1593
- https://doi.org/10.1101/gr.149200
Abstract
Apicomplexan protozoan parasites have complex life cycles that involve phases of asexual and sexual reproduction. Some genera have intermediate insect hosts, for example, Plasmodium spp. (the cause of malaria), but related genera such as Eimeria spp. (causative agents of coccidiosis in poultry) have a direct life cycle occurring in only a single host. Mechanisms that regulate the life cycles of apicomplexan parasites are unknown, but the intracellular growth of avian Eimeria spp. is easily shortened by serial selection for the first parasites to complete the transition from asexual to sexual reproduction (to yield so-called precocious lines). To investigate the genetic basis of such an abbreviated life cycle, we have used the species E. tenella and analyzed the inheritance of 443 polymorphic DNA markers in 22 recombinant cloned progeny derived from a cross between parents that had selectable phenotypes of precocious development or resistance to an anticoccidial drug. The markers were placed in 16 linkage groups (which defined 12 chromosomes) and a further 57 unlinked groups. Two linkage groups showed an association (P = .0105) with the traits of precocious development or drug-resistance and were mapped to chromosome 2 (ca 1.2 Mbp) and chromosome 1 (ca 1.0 Mbp), respectively. The map provides a framework for further studies on the identification of genetic loci implicated in the regulation of the life cycle of an important protozoan parasite and a representative of a major taxonomic group.[A table with the segregation data is available as an online supplement at http://www.genome.org.]Keywords
This publication has 31 references indexed in Scilit:
- Eimeria tenella: infection with a single sporocyst gives a clonal populationParasitology, 1996
- A commercial vaccine for ovine toxoplasmosisParasitology, 1995
- A genetic locus on Plasmodium falciparum chromosome 12 linked to a defect in mosquito-infectivity and male gametogenesisMolecular and Biochemical Parasitology, 1995
- AFLP: a new technique for DNA fingerprintingNucleic Acids Research, 1995
- An RFLP map of the Plasmodium falciparum genome, recombination rates and favored linkage groups in a genetic crossMolecular and Biochemical Parasitology, 1992
- A molecular karyotype of Eimeria tenella as revealed by contour-clamped homogeneous electric field gel electrophoresisMolecular and Biochemical Parasitology, 1990
- Further investigation of the pathogenicity, immunogenicity and stability of precocious Eimeria acervulinaParasitology, 1983
- A comparative study of the biological activities of arprinocid and arprinocid-1-N-oxideMolecular and Biochemical Parasitology, 1980
- Some observations on the sexual differentiation of Eimeria tenella using single sporozoite infections in chicken embryosParasitology, 1976
- Genetic studies on Plasmodium chabaudi: recombination between enzyme markersParasitology, 1975