Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945
- 15 January 1995
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 125 (2-3) , 287-292
- https://doi.org/10.1016/0378-1097(94)00510-x
Abstract
The pac gene encoding the penicillin G acylase (PGA) of Bacillus megaterium ATCC 14945 has been cloned in Escherichia coli HB101 (proA, leuB) using a selective minimal medium containing phenylacetyl-L-leucine instead of L-leucine. The nucleotide sequence of this gene has been determined and contains an open reading frame of 2406 nucleotides. The deduced amino acid sequence shows significant similarity with other beta-lactam acylases. Although the PGA of B. megaterium is extracellular, the enzyme produced in E. coli appears to have a cytoplasmic localization.Keywords
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