In Vitro Studies of the Response of Hamster Lymphocytes to Phytohemagglutinin and Antigenic Stimulation

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Abstract
A semi-micromethod of in vitro culture of hamster lymphocytes was developed for studies of the immune response to cellular and soluble antigens and to microbial pathogens. The number of cells necessary for optimal stimulation in the 0.2-ml volume employed was 0.5 × 106 to 1.0 × 106; 20 to 60 cultures could be prepared from a single donor lymph node under these conditions. The response to phytohemagglutinin did not differ significantly from that described for several other laboratory species. A mixed lymphocyte reaction to human and other xenogeneic cells was demonstrated but allogeneic mixtures of Golden Syrian and MHA strains did not stimulate DNA synthesis. Responses to tuberculin-purified protein derivative and keyhole limpet hemocyanin were elicited in cultures from animals immunized with these antigens. Although cells from the mesenteric lymph node were employed for most experiments, similar reactivity was obtainable with peripheral blood lymphocytes.

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