Quantification of Polyacrylamide Gel Electrophoresis for Analysis of Whey Proteins

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Abstract
Polyacrylamide gel electrophoresis of whey proteins was quantified by standardization of the separation and staining procedure. During each electrophoresis experiment, a standard solution of whey proteins was separated and stained under the same conditions as the test material, thus proteins in the standard solution were subjected to identical processing conditions as the test samples. Densitometric scanning of the stained protein-containing gels followed by peak area determinations was carried out. By comparison with the standard peak areas, individual protein concentrations of the test samples were determined.