Periodic repression by the bHLH factor Hes7 is an essential mechanism for the somite segmentation clock
Open Access
- 3 June 2003
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 17 (12) , 1451-1456
- https://doi.org/10.1101/gad.1092303
Abstract
Hes7, a bHLH gene essential for somitogenesis, displays cyclic expression of mRNA in the presomitic mesoderm (PSM). Here, we show that Hes7 protein is also expressed in a dynamic manner, which depends on proteasome-mediated degradation. Spatial comparison revealed that Hes7 and Lunatic fringe (Lfng) transcription occurs in the Hes7 protein-negative domains. Furthermore, Hes7 and Lfng transcription is constitutively up-regulated in the absence of Hes7 protein and down-regulated by stabilization of Hes7 protein. Thus, periodic repression by Hes7 protein is critical for the cyclic transcription of Hes7 and Lfng, and this negative feedback represents a molecular basis for the segmentation clock.Keywords
This publication has 33 references indexed in Scilit:
- Transcriptional oscillation of Lunatic fringe is essential for somitogenesisGenes & Development, 2003
- Wnt3a Plays a Major Role in the Segmentation Clock Controlling SomitogenesisDevelopmental Cell, 2003
- Periodic Notch inhibition by Lunatic Fringe underlies the chick segmentation clockNature, 2003
- Oscillatory Expression of the bHLH Factor Hes1 Regulated by a Negative Feedback LoopScience, 2002
- Clock Regulatory Elements Control Cyclic Expression of Lunatic fringe during SomitogenesisDevelopmental Cell, 2002
- Periodic Lunatic fringe Expression Is Controlled during Segmentation by a Cyclic Transcriptional Enhancer Responsive to Notch SignalingDevelopmental Cell, 2002
- Vertebrate SomitogenesisAnnual Review of Cell and Developmental Biology, 2001
- Dynamic expression and essential functions of Hes7 in somite segmentationGenes & Development, 2001
- Cofactor Dynamics and Sufficiency in Estrogen Receptor–Regulated TranscriptionCell, 2000
- A gene that resuscitates a theory—somitogenesis and a molecular oscillatorTrends in Genetics, 1998