Purification and Characterization of Cytochrome 553 from the Chrysophycean Alga Monochrysis lutheri

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Abstract
Cytochrome 553, an electron carrier in photosynthesis and a functional analogue of cytochrome f of higher plants, was isolated from Monochrysis lutheri and purified by salt fractionation, chromatography, and isoelectric focussing. Absorption maxima occurred at 275, 320, 416, 523, and 553 nm in the reduced form. The α-absorption peak was symmetrical and had an extinction coefficient of 25.9 mM−1 cm−1. The molecular weight was 11 100 by equilibrium centrifugation, 12 400 from iron determinations, and 10 300 from the amino acid composition. The molecule consisted of one heme group and 91 amino acid residues including two cysteine residues and one each of histidine, arginine, tryptophan, methionine, and proline. Other physical properties measured were: the sedimentation coefficient, 1.3 S; the normal oxidation reduction potential, + 0.388 V; and the isoelectric point, pH 3.75.

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