Co-localization of cell surface receptors at high spatial resolution by single-particle fluorescence imaging
- 1 December 2003
- journal article
- conference paper
- Published by Portland Press Ltd. in Biochemical Society Transactions
- Vol. 31 (6) , 1453-1455
- https://doi.org/10.1042/bst0311453
Abstract
Dual-wavelength single-particle fluorescence imaging has been used to quantify the co-localization of receptors and/or ligands on cells by widefield microscopy. Methods for correction of chromatic aberration and identification of submicroscopic artefacts are presented, with data for the lipopolysaccharide/CD14 and MHC class II/CD74 systems.Keywords
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