Sequencing of 16S− 23S rRNA internal transcribed spacer and its application in the identification of Nocardia seriolae by polymerase chain reaction
- 23 October 2002
- journal article
- research article
- Published by Hindawi Limited in Aquaculture Research
- Vol. 33 (14) , 1195-1197
- https://doi.org/10.1046/j.1365-2109.2002.00778.x
Abstract
A method for the diagnosis of nocardiosis in yellowtail (Seriola quinqueradiata), using polymerase chain reaction (PCR), was developed in this study. Primers specific for Nocardia seriolae were synthesized based on the alignment of 16S−23S rRNA internal transcribed spacer region sequences of N. seriolae. The primers did not amplify specific PCR product from other fish pathogens. However, two and three fishes could be diagnosed as infected with N. seriolae by clinical signs and bacterial isolation. PCR amplification of N. seriolae by specific primers detected six infected fishes. Thus, the primers used in this study are useful in detecting nocardiosis in fish.Keywords
This publication has 2 references indexed in Scilit:
- Nocardia seriolae sp. nov. Causing Nocardiosis of Cultured FishInternational Journal of Systematic and Evolutionary Microbiology, 1988
- Nocardial infection in cultured yellowtails (Seriola quinqueruiata and S. purpurascens)—IFish Pathology, 1968