A simple test for adaptation of soil microorganisms to the degradation of the insecticide Carbofuran

Abstract
Soils in which the microbial community had adapted to degrade carbofuran could be readily distinguished from unadapted soils by incubating a small quantity of the soil in an aqueous buffer containing 10 ppm carbofuran for 7 days. Adapted soils were characterized by a significant reduction (>90%) of the carbofuran concentration and a simultaneous increase in concentration of the stable phenolic hydrolysis product as determined by direct HPLC analysis of the solution. A simple colour test for the phenol also provided a measure of the activity of the soil toward carbofuran.

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