Purification and partial characterization of rat liver soluble catechol‐ O‐methyltransferase
- 7 May 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 264 (1) , 95-99
- https://doi.org/10.1016/0014-5793(90)80774-d
Abstract
The rat liver soluble catechol‐O‐methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography.Keywords
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