Apparent dye-dilution curves produced by injection of transparent solutions

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Abstract
In studies on dogs, blood was sampled as for the recording of dye-dilution curves through monochromatic densitometers. Injection of hypertonic media into the superior vena cava caused transient increases in the optical density of blood in the pulmonary and femoral arteries so as to produce apparent “dye” curves. The injection of hypotonic and hemolyzing media produced negative deflections, representing decreases in the optical density of blood, as did brief ventilation with 5% and 50% carbon dioxide in oxygen. Similar changes were demonstrated in vitro. They were compensated for in vivo by the use of a dichromatic densitometer. The changes observed are attributed to altered physical properties of the erythrocytes, which consequently change their light-transmitting and light-reflecting characteristics. Such nonspecific changes in the optical density of blood may constitute an important source of error in monochromatic densitometry done on whole blood. Submitted on January 11, 1961