Requirement of poly(rC) binding protein 2 for translation of poliovirus RNA.
- 1 August 1997
- journal article
- Vol. 71 (8) , 6243-6
Abstract
Poly(rC) binding protein 2 (PCBP2) is one of several cellular proteins that interact specifically with a major stem-loop domain in the poliovirus internal ribosome entry site. HeLa cell extracts subjected to stem-loop IV RNA affinity chromatography were depleted of all detectable PCBP2. Such extracts were unable to efficiently translate poliovirus RNA, although extracts recovered from control columns of matrix unlinked to RNA retained full translation activity. Both translation and production of infectious progeny virus were restored in the PCBP2-depleted extracts by addition of recombinant PCBP2, but not by PCBP1, which is a closely related member of the protein family. The data show that PCBP2 is an essential factor, which is required for efficient translation of poliovirus RNA in HeLa cells.This publication has 23 references indexed in Scilit:
- Translation and Replication Properties of the Human Rhinovirus Genomein Vivoandin VitroVirology, 1997
- Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: identification by automated liquid chromatography-tandem mass spectrometry.Proceedings of the National Academy of Sciences, 1996
- Detection and Characterization of a 3′ Untranslated Region Ribonucleoprotein Complex Associated with Human α-Globin mRNA StabilityMolecular and Cellular Biology, 1995
- Anatomy of the Poliovirus Internal Ribosome Entry SitePublished by Springer Nature ,1995
- Internal initiation of translation of picornavirus RNAsMolecular Biology Reports, 1994
- Tissue specific expression and cDNA structure of a human transcript encoding a nucleic acid binding [oligo(dC)] protein related to the pre-mRNA binding protein KNucleic Acids Research, 1994
- A cytoplasmic 57-kDa protein that is required for translation of picornavirus RNA by internal ribosomal entry is identical to the nuclear pyrimidine tract-binding protein.Proceedings of the National Academy of Sciences, 1993
- A cellular protein that binds to the 5'-noncoding region of poliovirus RNA: implications for internal translation initiation.Genes & Development, 1989
- Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNANature, 1988
- Purified lupus antigen la recognizes an oligouridylate stretch common to the 3′ termini of RNA polymerase III transcriptsCell, 1984