Studies on plant cell fusion: Effects of dextran and pronase E on fusion.

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Abstract
To clarify conditions of plant cell fusion, dextrans with various molecules were examined with protoplasts [Brassica pekinensis cv. Sendai Hakusai] of different developmental stages. Dextrans were not toxic for protoplasts and high molecular dextran can possibly induce both protoplast aggregation and fusion by adding an electrolyte, such as inorganic salts. Fusion occurred among young leaf protoplasts more frequently than among those of old leaf protoplasts. Fusion frequencies were enhanced by pronase E treatment of protoplasts, even among old leaf protoplasts. After stopping pronase E treatment, fusion frequencies decreased by degrees. Protein accumulated during the aging of cells on the plasma membrane and evidently played a part in preventing plant cells from fusion.

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