ANISAKIS AND EOSINOPHIL .1. DETECTION OF A SOLUBLE FACTOR SELECTIVELY CHEMOTACTIC FOR EOSINOPHILS IN EXTRACT FROM ANISAKIS LARVAE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 120  (3) , 745-749
Abstract
Studies were undertaken to determine if Anisakis larva itself has the ability to attract eosinophils. Extracts from Anisakis larvae were examined for neutrophil and eosinophil chemotactic activities with in vivo and in vitro assay systems. When the soluble extract was injected intradermally into normal guinea pigs, a profound accumulation of eosinophils was observed at the injection site. The cells started to accumulate at the site within 1 h and the number of eosinophils at the site reached its peak at 8 h after the injection of the extract. Such eosinophilic accumulation was enhanced in a dose-response fashion over the range of 0.5-50 .mu.g protein. The strong chemotactic activity of the Anisakis extract for eosinophils was confirmed when in vitro chemotaxis assays were performed with Boyden chemotactic chambers. No chemotactic activity for neutrophils was found at those concentrations of the extract with which the eosinophil effect was observed. The factor described here, in addition to the various known immunologic factors, may play an important role in the development of eosinophilia in anisakiasis.

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