Conjugation of disodium 6-chloro-4-oxo-10-propyl-4H-pyrano[3,2-g]quinoline-2,8-dicarboxylate with glutathione in the rat

Abstract
Disodium 6-chloro-4-oxo-10-propyl-4H-pyranol[3,2-g]quinoline-2,8-dicarboxylate (I) [an antiallergic agent] is conjugated with glutathione (GSH) both in vitro and in vivo by nucleophilic replacement of the chlorine substituent in (I). The reaction is catalyzed by rat-liver supernatant. The GSH conjugate is the principal excretory product in the rat bile after i.v. administration of (I), accounting for .apprx. 60% of the dose and 95% of the biliary excretion. Its structure was determined by synthesis and spectroscopic analysis.