Facultatively anaerobic nitrogen-fixing bacteria from the marine environment
- 1 January 1974
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 20 (1) , 59-64
- https://doi.org/10.1139/m74-010
Abstract
Anaerobic nitrogen fixation in the dark was determined in a natural marine community, developed in a laboratory model ecosystem, at the Oregon State University Marine Science Center in Newport, Oregon. Fixation occurred at a rate of 10 nmol C2H4/cm2 per 24 hours. Two different marine N2-fixing bacteria were isolated from seawater and from sediment at the Oregon coast. These bacterial isolates were identified as Klebsiella pneumoniae and Enterobacter aerogenes. Within a growth period of 100 h K. pneumoniae fixed 3.6 mmol N2 per liter and E. aerogenes fixed 1.2 mmol N2 per liter. The influence of seawater on N2-fixation activity of these bacteria was compared with that of bacteria from a non-marine origin. The nitrogenase activity of a crude cell-free extract of E. aerogenes was dependent upon ATP and Na2S2O4 and proceeded at a rate of 3.9 nmol C2H4 per minute per milligram protein.Keywords
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