Differential expression of cGMP‐inhibited cyclic nucleotide phosphodiesterases in human hepatoma cell lines
- 15 July 1996
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 390 (1) , 29-33
- https://doi.org/10.1016/0014-5793(96)00410-3
Abstract
PDE3 or cGMP‐inhibited cyclic nucleotide phosphodiesterase (cGI PDE) activity was detected in homogenates of HepG2, Hep3B and HuH7, but not SK‐Hep‐1, human hepatoma cells. In HepG2 and Hep3B cells PDE3 activity was found predominantly in particulate fractions; in HuH7, in both particulate and supernatant fractions. cDNAs encoding two human PDE3s (an ‘adipocyte’ type, HcGIP1),and a ‘cardiovascular’ type, HcGIP2) have been cloned. HcGIP1 cDNA hybridized strongly with poly(A)+ RNA species from HepG2 and Hep3B. Both HcGIPI and HcGIP2 mRNAs were expressed in Hep3B and HuH7 cells. The nucleotide sequence of an ∼300‐bp cDNA fragment, isolated after RT‐PCR cloning from HepG2 RNA, was identical to a sequence within the conserved domain of HcGIP1 cDNA, consistent with the presence of HcGIPI mRNA in HepG2 cells.Keywords
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