INHIBITION OF GLUCOSE-INDUCED INSULIN RELEASE BY SOMATOSTATIN IN ISOLATED PERFUSED RAT PANCREAS - ACTION OF CYCLIC-AMP, GLUCAGON AND GLIBENCLAMIDE

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 32  (3) , 211-216
Abstract
Insulin release in the perfused isolated rat pancreas was measured after stimulation with 16.5 mM glucose with and without somatostatin (cyclic form, 100 ng/ml) in the medium. A complete blockage of the typical biphasic pattern of insulin release occurred with somatostatin in the medium. Such blockage was abolished when c[cyclic AMP]AMP (2.5 mM) and a 0.5 ml solution of glucagon (1 mg/ml) were continuously perfused for 20-min periods and for 30-s periods correspondently. It did not take place when glibenclamide (HB-419) was perfused for a 20-min period at a rate of 10 .mu.g/ml. The adenylate cyclase dependent mechanisms of glucose-induced insulin release are probably involved in the inhibition of the glucose-induced insulin secretion by somatostatin.