A chloramphenicol-streptomycin-resistance plasmid from a clinical strain of Staphylococcus sciuri and its structural relationships to other staphylococcal resistance plasmids

15 August 1991

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 82 (3) , 319-322
https://doi.org/10.1016/0378-1097(91)90281-e

Abstract

A 5.1-kb plasmid, designated pSCS12, isolated from a naturally occurring Staphylococcus sciuri conferred resistance to chloramphenicol (Cm^R) and streptomycin (Sm^R). Restriction endonuclease analyses of pSCS12 revealed partial structural homologies to the Cm^R-plasmids pC221 from S. aureus and pSCS1 from S. intermedius, to the Sm^R-plasmids pSAI-1 from S. hyicus and pS194 from S. aureus, as well as to the Cm^R/Sm^R plasmid pSK68 from S. aureus. Southern-blot hybridization with specific Cm^R- and Sm^R-gene probes confirmed these similarities and allowed the mapping of the Cm^R- and Sm^R-determinants in the S. sciuri plasmid pSCS12. These observations lead to the suggestion that Cm^R/Sm^R-plasmids, such as pSCS12, may have evolved from Cm^R- and Sm^R-plasmids by interplasmidic recombination.

Keywords

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