Immunocytochemical localization of wheat prolamins in the lumen of the rough endoplasmic reticulum
- 1 November 1991
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Botany
- Vol. 69 (11) , 2574-2577
- https://doi.org/10.1139/b91-320
Abstract
Antibodies raised against gliadins, the alcohol-soluble proteins of wheat (Triticum aestivum L.) seeds, were used to localize gliadins within the lumen of the endoplasmic reticulum. Endosperm cells at 20 days after anthesis contain extensive rough endoplasmic reticulum that is fragmented and dilated. The dilated endoplasmic reticulum encloses aggregates of proteinaceous material that reacts strongly with gliadin-specific antibodies. Key words: gliadins, immunocytochemistry, protein A – gold, rough endoplasmic reticulum, wheat.Keywords
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