Fractionation of Some Bovine Whey Proteins by Recycling Gel Filtration on a Large Scale
- 1 January 1975
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 5 (2) , 131-146
- https://doi.org/10.1080/00327487508061566
Abstract
Fractionation of bovine whey concentrate was performed by gel filtration on Sephadex G-75 both on a laboratory scale and on a large scale. By a recycling procedure an improved separation was obtained and the whey proteins were resolved into four fractions in the weight ratio 3:12:1:4. The fractions were analysed by polyacrylamide gel (PAG) electrophoresis and the apparent molecular weights were determined by thin layer gel chromatography (TLG) and by sodium dode-cylsulfate (SDS) gel electrophoresis.Keywords
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