Improved High Pressure Liquid Chromatographic Method for Phenylpropanolamine in Human Plasma
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 16 (9) , 693-699
- https://doi.org/10.1080/00032718308065205
Abstract
A high-pressure liquid chromatographic analysis of phenylpropanolamine in plasma following extraction, back extraction and pre-column derivitization with O-phthalaldehyde is presented. The method is improved by the use of phenylethanolamine as internal standard. Using fluorescence detection, the method is sufficiently sensitive to quantitate 5 ng/ml in 0.5 ml plasma with a standard error of estimate of 2.7 ng/ml when calibrated over the O to 240 ng/ml range. Analysis of over 2000 clinical samples have shown the method to be highly specific and reliable.Keywords
This publication has 1 reference indexed in Scilit:
- High-pressure Liquid Chromatographic Analysis of Phenylpropanolamine in Human Plasma following Derivatization with O-PhthalaldehydeJournal of Pharmaceutical Sciences, 1981