Quantitative Determination of Polar Lipids on Thin Layer-FID Chromatography

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Abstract
In the analysis of the phospholipid mixtures which contained phosphatidyicholine, cardiolipin, phosphatidylethanolamine, sphingomyelin and lysophosphatidylcholine, the relative responses for quantitative thin layer chromatography combined with a hydrogen flame ionization detector (thinchrography) represented a good coincidence with the theoretical responses based on weight per cent. Meanwhile, it was also found that the thinchrograph is a useful tool for the estimation of the relative amounts of nonpolar lipid to polar lipid or neutral lipid to phospholipid and glycolipid in the total lipid extract.

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