The Phosphorylation of Proteins in Hippocampal Slices: Effects of Noradrenaline and of Pretreatment with Kainic Acid

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Abstract
Rat hippocampal slices were incubated in the presence of [32P]Pi, and protein phosphorylation was examined by means of sodium dodecyl sulfate-gel electrophoresis. Incubation for at least 30 min with 300 .mu.Ci of [32P]Pi/brain slice gave rise to the phosphorylation of 8-10 protein bands. Most of these bands showed enhanced phosphorylation in response to noradrenaline [norepinephrine]. The basal phosphorylation of kainic acid-pretreated hippocampal slices was enhanced 2- to 3-fold compared with controls. There was also an additional increase in kainic acid-pretreated slices in the response to noradrenaline. 8-Br-cAMP and phosphodiesterase inhibitors, such as papaverine or isobutylmethylxanthine, had no effect on the phosphorylation patterns.