Langerhans Cells in Squamous Exocervical Carcinoma: A Quantitative and Ultrastructural Study

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Abstract
The gross morphology, density, distribution, and ultra-structure of the Langerhans cell (LC) in the human exocervix in cases of cervical intraepithelial neoplasia (CIN) and incipient carcinoma were investigated. Two zinc-iodide-osmium (ZIO) procedures were applied to epithelial sheets and to tissue sections for light and electron microscopy. The ZIO methods as well as conventional electron microscopy revealed the presence of LCs in CIN, in the invasive prongs, and in the lym-phoid infiltrate of the chorion. The epithelial sheets demonstrated a LC density 3.5 times higher than we have previously reported for the normal exocervix. The region around the external os displayed the highest LC density and the most advanced grades of CIN. The LCs were classified into six types according to the number of processes and secondary branches. The most ramified types were more abundant in the neo-plastic exocervix than in the normal exocervix. The LC in all locations of the exocervix with carcinoma showed hypertrophy of the rough and smooth endo-plasmic reticula and a large number of filaments. The RER displayed dilated cisternae containing electron-dense material. Specialized contacts between LCs and lymphocytes were an outstanding finding. These findings suggest that the LC might play an important role in the neoplastic process.