RNA-directed RNA polymerases from healthy and from virus-infected cucumber.
- 1 April 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (8) , 2383-2386
- https://doi.org/10.1073/pnas.83.8.2383
Abstract
Much work has been done on the isolation, purification, and characterization of the RNA-directed RNA polymerse (EC 2.7.7.48) of cucumber mosaic virus (CMV)-infected cucumbers. Unifected plants were reported to have no such enzyme, but we recently detected low levels of the activity in cucumber. Since tobacco and cowpea contain such an enzyme that is variably increased in amount by various virus (as well as viroid) infections, we assumed that this would also be the case upon CMV infection of cucumber. However, further purification and characterization of the RNA-directed RNA polymerases from healthy and from infected cucumber suggests that these are different enzymes. The presumed CMV replicase was obtained pure and consists of a major polypeptide of Mr 100,000 and minor components of Mr 110,000 and about 10,000. The Km is 5 .mu.M ([3H]GTP) when tobacco mosaic virus RNA is used as template.This publication has 19 references indexed in Scilit:
- Purification and characterization of polynucleotide phosphorylase from cucumberProceedings of the National Academy of Sciences, 1985
- RNA-dependent RNA polymerases of plantsProceedings of the National Academy of Sciences, 1983
- Highly active template-specific RNA-dependent RNA polymerase from barley leaves infected with brome mosaic virus.Proceedings of the National Academy of Sciences, 1979
- Extensive purification of the cucumber mosaic virus-induced RNA replicaseVirology, 1979
- Characterization of the RNA-dependent RNA polymerase of tobacco leaves.Journal of Biological Chemistry, 1979
- RNA-dependent RNA polymerases in uninfected and tobacco mosaic virus-infected tobacco leaves: Viral-induced stimulation of a host polymerase activityVirology, 1978
- RNA-dependent RNA polymerases in uninfected and in alfalfa mosaic virus-infected tobacco plantsVirology, 1977
- RNA polymerase from tobacco necrosis virus-infected and uninfected tobaccoVirology, 1977
- RNA polymerase from tobacco necrosis virus infected and unifected tobacco. Purification of the membrane-associated enzymeVirology, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970