Morphological Changes in Human Polymorphonuclear Leukocytes Induced by C3a in the Presence and Absence of Cytochalasin B
- 1 January 1982
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 69 (1) , 62-67
- https://doi.org/10.1159/000233147
Abstract
Treatment of human peripheral polymorphonuclear leukocytes (PMNs) with C3a leads to profound changes in cell morphology, along with the limited secretion of lysozyme. Simultaneous addition of C3a and cytochalasin B enhances the release of lysosomal enzymes and renders the cell morphology similar to that seen when either C5a or n-formyl peptides are added to PMNs in the presence of cytochalasin B. The morphological response of PMNs treated with C3a is considerably more heterogeneous than that induced by either C5a or n-formyl-methionyl-leucyl-phenylalanine, indicating that some, but not all, PMNs have receptors for C3a.Keywords
This publication has 15 references indexed in Scilit:
- Degranulating stimuli increase the availability of receptors on human neutrophils for the chemoattractant f-met-leu-phe.The Journal of Immunology, 1980
- Anaphylatoxin-induced histamine release with human leukocytes: studies of C3a leukocyte binding and histamine release.Journal of Clinical Investigation, 1979
- Anaphylatoxin from the fifth component of porcine complement. Purification and partial chemical characterization.Journal of Biological Chemistry, 1979
- Secretory Responses of Human Neutrophils: Exocytosis of Specific (Secondary) Granules by Human Neutrophils during Adherence in vitro and during Exudation in vivoThe Journal of Immunology, 1979
- Selective neutrophil desensitization to chemotactic factors.The Journal of cell biology, 1979
- CHEMOTACTIC RESPONSE TO HUMAN C3A AND C5A ANAPHYLATOXINS .1. EVALUATION OF C3A AND C5A LEUKOTAXIS INVITRO AND UNDER SIMULATED INVIVO CONDITIONS1978
- Complement (C5-a)-induced granulocyte aggregation in vitro. A possible mechanism of complement-mediated leukostasis and leukopenia.Journal of Clinical Investigation, 1977
- Demonstration of a receptor on rabbit neutrophils for chemotactic peptidesBiochemical and Biophysical Research Communications, 1977
- Measurement of chemotaxis of human polymorphonuclear leukocytes in filters by counting the number of cells in a single plane and comparison with leading front methodJournal of Immunological Methods, 1976
- PARTIAL CHARACTERIZATION OF HUMAN C5A ANAPHYLATOXIN .1. CHEMICAL DESCRIPTION OF CARBOHYDRATE AND POLYPEPTIDE PORTIONS OF HUMAN C5A1976