Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Metabolically Engineered Escherichia coli Strains
- 23 November 2000
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 2 (1) , 248-254
- https://doi.org/10.1021/bm000105u
Abstract
Recombinant Escherichiacoli strains harboring a plasmid containing a novel artificial polyhydroxyalkanoate (PHA) operon consisting of the Aeromonas PHA biosynthesis related genes and Ralstoniaeutropha reductase gene were developed for the production of poly(3-hydroxybutyrate-co-hydroxyhexanoate) [P(3HB-co-3HHx)] from dodecanoic acid. By applying stepwise reduction of dissolved oxygen concentration (DOC) during the fermentation, the final dry cell weight, PHA concentration, and PHA content of 79 g/L, 21.5 g/L, and 27.2 wt %, respectively, were obtained in 40.8 h, which resulted in the PHA productivity of 0.53 (g/L)/h. The 3HHx fraction slowly increased during the fed-batch culture to reach a final value of 10.8 mol %. The 3HHx fraction in the copolymer could be increased by 3-fold when the Aeromonashydrophilaorf1 gene was coexpressed with the PHA biosynthesis genes.Keywords
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