Abstract
A total of 447 cervical or vaginal specimens [human] were inoculated in parallel onto peptone-starch-dextrose (PSD) and Columbia colistin (10 mg/ml)-nalidixic acid (15 .mu.g/ml) (CNA) agar and were incubated for 48 h at 35.degree. C with 2-10% CO2. Of the cultures, 100 (22.4%) were positive for H. vaginalis; 48 were recovered from both PSD and Columbia CNA agar, 5 from PSD only, and 47 from Columbia CNA agar only (P < 0.001). On Columbia CNA agar, 76 of the isolates were detected after 24 h of incubation, and the remainder were detected within 4 days of incubation.