Organisation of Genes for Ribosomal RNA in Physarum polycephalum

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Abstract
Physarum polycephalum nucleolar satellite DNA has been analysed by restriction enzyme digests and hybridisation to ribosomal RNA. The nucleolar DNA is isolated as molecules of molecular weight 39 × 106. The positions of sites of endo R · EcoRl and endo R · HindIII digestion have been determined accurately in the whole molecule. The endo R · EcoRI sites and one endo R - Hind III site are within the 26-S rRNA complementary sequence and the other endo R · HindIII site is within the 19-S rRNA complementary sequence. The sites are arranged symmetrically about the centre of the molecule as if it were a palindrome.