Isolation and functional expression of pituitary peptidylglycine α‐amidating enzyme mRNA
- 3 September 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 269 (2) , 319-323
- https://doi.org/10.1016/0014-5793(90)81184-p
Abstract
We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.Keywords
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