Isolation and identification of a novel cDNA that encodes human yrdC protein
- 1 March 2003
- journal article
- research article
- Published by Springer Nature in Journal of Human Genetics
- Vol. 48 (4) , 164-169
- https://doi.org/10.1007/s10038-002-0001-3
Abstract
In the course of detecting the interaction protein of RBBP10 by yeast two-hybridization, we isolated a novel cDNA that encodes a putative human protein with yrdC domain. It is named human yrdC protein. Because the cDNA contains an open reading fragment (ORF) without a 5′ in- frame stop codon, 5′ RACE and 3′ RACE were proceeded to produce the full-length cDNA. An 1825 bp cDNA was isolated from human placenta, which encodes a putative protein of 279 amino acids. The protein contains a sua5-yciO-yrdC domain. Blast analysis against the human genome database of Genbank revealed that the gene contains five exons, and assigned the gene to human chromosome 1p34.2. A transcript about 2.5 kb is ubiquitously expressed in human tissues. The gene is highly conserved during evolution.Keywords
This publication has 15 references indexed in Scilit:
- The structure of the yrdC gene product from Escherichia coli reveals a new fold and suggests a role in RNA bindingProtein Science, 2000
- A retinoblastoma-binding protein that affects cell-cycle control and confers transforming abilityNature Genetics, 1998
- A putative helicase, the SUA5, PMR1, tRNALys1 genes and four open reading frames have been detected in the DNA sequence of an 8.8 kb fragment of the left arm of chromosome VII of Saccharomyces cerevisiae.1996
- A putative helicase, theSUA5, PMR1, tRNALys1 genes and four open reading frames have been detected in the DNA sequence of an 8·8 kb fragment of the left arm of chromosome VII ofSaccharomyces cerevisiaeYeast, 1996
- Extragenic suppressors of a translation initiation defect in the cyc1 gene of Saccharomyces cerevisiaeBiochimie, 1991
- Dimers, leucine zippers and DNA-binding domainsTrends in Genetics, 1990
- A human homologue of the yeast GST1 gene codes for a GTP-binding protein and is expressed in a proliferation-dependent manner in mammalian cells.The EMBO Journal, 1989
- A conserved amino acid sequence around Arg−68 of Artemia elongation factor 1α is involved in the binding of guanine nucleotides and aminoacyl transfer RNAsBiochimie, 1987
- EUKARYOTIC PROTEIN SYNTHESISAnnual Review of Biochemistry, 1985
- Eukaryotic Protein SynthesisAnnual Review of Biochemistry, 1985