A volumetric method for assessing Giardia inactivation

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Abstract
Based on these tests, the results of the proposed volumetric method—which has the advantage of reducing labor requirements—were indistinguishable from those of the standard method.Because the standard method of determining Giardia inactivation in disinfection tests—which involves counting dead and living organisms—can be extremely tedious, an alternative method, whereby the concentration of only viable trophozoites is estimated, is being sought. This article describes the development of such a volumetric method and its verification by comparing the results obtained with the standard and volumetric methods. This work demonstrates that the two methods yield equivalent results—at least for the disinfectants and waters examined. Whether this equivalence operates for larger‐scale concentration techniques and for different waters, disinfectants, and organisms remains open to further research.