Primary Tissue Culture Explants of Human Breast Cancer1

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Abstract
Primary monolayer cultures of epithelial cells from human breast cancer could be established with some degree of regularity in at least 50% of attempts with the use of biopsy specimens. Epithelial monolayers could occasionally be maintained up to 120 days. Serial cell lines were not developed from these primary cultures. Although a variety of cell dispersion methods are available, the use of collagenase to prepare an initial cell suspension from finely minced tissue appeared to be as good as any method. Commercially available media, such as RPMI 1640, McCoy's 5a, or NCTC 109 with 20% fetal calf serum, gave as good results as a more complex medium involving additives, such as insulin, hydrocortisone, estrogens, or extra glucose.

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