Synthesis of new hydrophilic microspheres: Optimized carriers for microparticle‐enhanced nephelometric immunoassays

Publisher Website
Google Scholar
Abstract
To optimize antigen–antibody reactions, we have synthesized chemically well-defined hy-drophilic microspheres. Proteins or haptens were covalently linked to these carriers. When the microsphere conjugates were agglutinated by the corresponding antiserum, the size of the complex artificially increased during the immunological reaction. After optimizing var-ious parameters such as the hydrophilic character, repulsion charges, and amount of antigen coupled to the microspheres, we developed a rapid and sensitive immunoassay based on laser light scattering by the complexes.