INFLUENCE OF BRONCHOALVEOLAR WASHING SUPERNATANTS AND STIMULATED LYMPHOCYTE SUPERNATANTS ON UPTAKE OF PASTEURELLA-HAEMOLYTICA BY CULTURED BOVINE ALVEOLAR MACROPHAGES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (3) , 443-446
Abstract
Bovine alveolar macrophages cultured for 12 h with normal bovine bronchoalveolar washing supernatants (BWS) showed impaired phagocytosis of P. haemolytica when compared with phagocytosis in control cultures. Such impairment was not observed when another gram-negative bacillus, Yersinia enterocolitica, was used. The BWS from calves inoculated s.c. or intrabronchially with P. haemolytica inhibited uptake of P. haemolytica when BWS were incubated with the bacteria prior to addition to macrophage cultures. Serum from these calves had pronounced opsonizing activity. Supernatants from stimulated and non-stimulated bovine peripheral blood lymphocyte cultures inhibited phagocytosis of P. haemolytica.