Extraction of high quality DNA from bloodstains using diatoms
- 1 May 1995
- journal article
- research article
- Published by Springer Nature in International journal of legal medicine
- Vol. 108 (3) , 154-156
- https://doi.org/10.1007/bf01844828
Abstract
A simple method is described for the extraction of high quality DNA for PCR amplification. The DNA was extracted by using Chelex-100 ion exchange resin or a special cell lysis buffer containing proteinase K. For further purification the DNA was bound to silica in the presence of a chaotrophic agent. Hence it is possible to unlimitedly wash the bound DNA and inhibitory substances are removed. By using diatoms as a source of silicates, this method is very economical and can therefore be used as a routine method.Keywords
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