Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin
Open Access
- 25 October 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 333 (1-2) , 197-202
- https://doi.org/10.1016/0014-5793(93)80404-i
Abstract
The mechanism by which iron‐storage proteins take up and oxidise iron(II) is not understood. We show by rapid‐kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron‐storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.Keywords
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