This article reports on the use of are troviral vector containing the enhanced green fluorescent protein gene (EGFP)

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Abstract
This article reports on the use of a retroviral vector containing the enhanced green fluorescent protein gene (EGFP), which has been used to transfect Daoy medulloblastoma cells in vitro and then to follow the invasive process when these cells were subsequently implanted into the brain of nude mice. This is a potentially interesting application of fluorescence technology, particularly the innovative use of molecular constructs containing EGFP, to determine the extent of invasion in tumor models. One could anticipate that this fluorescence tagging, in combination with three-dimensional imaging of tumors and invasive cells obtained using fluorescence confocal laser scanning microscopy, may yield very interesting results. Future developments of this technology may also provide important information regarding the coexpression pattern of genes involved in the invasive process, as well as detailing the temporal expression of these genes. It may have applicability both in this model of pediatric medulloblastoma and in other models...

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