Synteny‐mapping horse microsatellite markers using a heterohybridoma panel

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Abstract
Summary: A panel of horse‐mouse heterohybridoma cells was tested for genetic markers using biochemical and polymerase chain reaction‐(PCR‐) based tests. Biochemical markers included phospho‐glucomutase (PGM), glucose phosphate isomerase (GPI) and 6‐phosphogluconate dehydrogenase (PGD). Markers detected using PCR‐based tests included microsatellite markersHTG2–15, HMS 1–3, 5–8, VHL20, ECA2and genes for equine major histocompatibility geneELA‐DRA, tumour necrosis factor alpha (TNFA) and transferrin. The results were analysed for correlation and concordance. Based on the results, five synteny groups were identified, specifically betweenELA‐DRA, TNFA, HMS5andHTG5; betweenHTG3andHTG13; betweenHTG4, HTG8andHMS3; betweenHTG6andHMS1; and betweenHTG7, HTG9andHMS6.Evidence was also found for synteny betweenHTG12, HMS7andECA2, however, confirmation requires further testing. Cytogenetic evaluation of the cell lines making up the panel indicated that large metacentric chromosomes were preferentially lost or tended to break at the centromere. Consequently, the results from this analysis can be used to identify synteny, but not to exclude synteny.