The representativeness of extracted samples and the efficiency of extraction of protein from the fresh leaves of plants; and some partial analyses of the whole proteins of leaves

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Abstract
Samples of protein extracted from fresh plant leaves by various methods all involving maceration with solvents followed by removal of cell debris, have been compared in composition with the whole proteins of the leaves and with those contained in the leaf residues. Amide, tyrosine and tryptophan contents and sulphur distributions (cystine + cysteine and methionine contents) were estimated for this purpose. If due precautions are not taken there is a tendency preferentially to leave protein associated with the "granule" fraction (nuclei, plastids, mitachondria, etc.) in the residues. Most of the protein of this fraction passes into soln. in mildly alkaline buffers (pH 9) on the addition of miscible lipoid solvents (alcohol, ether), and their use in the preparation of purer samples of reasonably representative protein is demonstrated. The partial analyses of the whole proteins of the leaves provided no evidence of variation in composition with the age of the leaves or the manurial and climatic conditions or locality of growth, but composition may vary with plant species.

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