Comparative Determination of Bacitracin by HPLC and Microbiological Methods in Some Pharmaceuticals and Feed Grade Preparations

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Abstract
In this text, reverse phase HPLC method for separation of bacitracin is described. By selection of optimum chromatographic parameters in isocratic elution, the procedure for quick simple and precise determination of the most important active bacitracin components A, B1 and B2 was obtained. In relation to microbiological determination it was concluded that selective HPLC method gave considerably more useful analytical information on properties and origin of bacitracin samples. Intermethod differences in analysing bacitracin feed grade preparations, can be explained by specific ingredients in this type of samples and less selective microbiological method. Described HPLC method expresses certain advantages over some other published HPLC methods and it is suitable for quantitative and qualitative determinations of bacitracin in pharmaceuticals and feed grade preparations. In stability studies, it is possible to observe, in this way, the appearance of bacitracin F, the main degradation product of bacitracin. It was also found out that component of bacitracin B1 is not homogeneous. Under specific conditions it can be separated in two different submits, and we sign them as B', and B'