Renal Chromophobe Cell Carcinoma: Limitations of Paraffin-Embedded Tissue
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in Ultrastructural Pathology
- Vol. 17 (5) , 529-536
- https://doi.org/10.3109/01913129309041304
Abstract
Chromophobe cell carcinoma of the kidney is distinguished from other renal cortical tumors by the ultrastructural presence of numerous cytoplasmic vesicles of unknown composition. Failure to detect vesicles in paraffin-embedded tissue in the archival material of 10 putative chromophobe cell tumors stimulated an investigation into the effects of fixatives and dehydrating solvents on vesicle integrity. We found that although vesicles are removed by dehydrating agents during paraffin embedding, osmium tetroxide postfixation prevents vesicle loss during dehydration for electron microscopy. We conclude that paraffin-embedded tissue is useless for histochemical studies of vesicle composition and cannot be employed for the ultrastructural confirmation of a histologic diagnosis of chromophobe cell carcinoma since the intracellular vesicles are no longer present.Keywords
This publication has 9 references indexed in Scilit:
- Chromophobe Cell CarcinomaThe American Journal of Surgical Pathology, 1990
- The human chromophobe cell renal carcinoma: Its probable relation to intercalated cells of the collecting ductVirchows Archiv B Cell Pathology Including Molecular Pathology, 1988
- Chromophobe cell renal carcinoma and its variants—a report on 32 casesThe Journal of Pathology, 1988
- The formaldehyde-fixed and paraffin-embedded tissues for diagnostic transmission electron microscopy: A retrospective and prospective studyHuman Pathology, 1987
- Sarcomatoid renal tumors. Clinicopathologic correlation of three casesCancer, 1987
- The Ultrastructure and Immunocytochemistry of Renal Cell CarcinomaUltrastructural Pathology, 1987
- Human chromophobe cell renal carcinomaVirchows Archiv B Cell Pathology Including Molecular Pathology, 1985
- New Techniques Rapid Deparaffinization for Electron MicroscopyUltrastructural Pathology, 1984
- Osmium TetroxidePublished by Elsevier ,1981