Production of cellulase and protein from barley straw by Trichoderma viride

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Abstract
The cellulase production by two strains of the cellulolytic fungus Trichoderma viride was examined. The fungi were grown on different preparations of barley straw pretreated with NaOH under high pressure. The production of cellulases and microbial protein by the better strain (QM 9123) was investigated in an aerated 5‐liter fermenter under varying stirring rates (200‐350 rpm) and straw concentrations (1–2%). The pH was kept between 3.5 and 4.5. The growth of the fungus was followed by measuring the quantity of CO2 produced and the cell protein. After 2–6 days growth ceased, the lag phases lasting 0–2 days, increasing with increasing straw concentrations. The maximum enzyme yields were reached after 4–10 days. The protein content of the product was 21–26% and up to 70% of the straw was utilized. The yield constants were calculated to be 0.40–0.56; of the same order as those which can be obtained by growing the fungus on glucose.

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