Molecular cloning of Moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome.
- 1 December 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (12) , 6314-6318
- https://doi.org/10.1073/pnas.76.12.6314
Abstract
The unintegrated circular DNA form of Moloney murine sarcoma virus (MSV) was cloned in bacteriophage .lambda.. Discrete deletions in the viral genome occurred during propagation of recombinant phage in Escherichia coli. Heteroduplex and restriction enzyme analyses indicated the deletion of tandemly repeated sequences within certain of the cloned MSV DNA inserts. Cloned MSV DNA was used to prepare a probe composed of its acquired cellular (src) sequences, shown previously to be necessary for MSV transformation. Analysis of EcoRI digests of normal mouse cellular DNA revealed the presence of a single 14-kilobase-pair fragment containing these sequences which lacked contiguity with endogenous type C helper viral information of the same cells. Thus, the sarcoma virus-specific sequences of MSV are represented within the normal mouse genome in a manner analogous to that of a cellular gene.This publication has 31 references indexed in Scilit:
- Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda.Proceedings of the National Academy of Sciences, 1979
- Restriction enzyme analysis of mouse cellular type C viral DNA: emergence of new viral sequences in spontaneous AKR/J lymphomas.Proceedings of the National Academy of Sciences, 1979
- In vitro synthesis of a 9 kbp terminally redundant DNA carrying the infectivity of moloney murine leukemia virusCell, 1979
- A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfectionCell, 1979
- Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA: Preparation and Screening of Shotgun CollectionsScience, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- In vitro packaging of a λ Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1Gene, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Cleavage map of linear mouse sarcoma virus DNA.Proceedings of the National Academy of Sciences, 1977
- A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.Proceedings of the National Academy of Sciences, 1967