CYCLOPHOSPHAMIDE INDUCED IN VIVO SISTER CHROMATID EXCHANGES (SCE) IN MUS MUSCULUS. I: STRAIN DIFFERENCES AND EMPIRICAL ASSOCIATION WITH RELATIVE CHROMOSOME SIZE
- 1 October 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Genetics and Cytology
- Vol. 24 (5) , 521-528
- https://doi.org/10.1139/g82-055
Abstract
The inducibility of sister chromatid exchanges (SCEs) by cyclophosphamide (CP) in bone marrow cells was evaluated in vivo in the three genetic strains of mice (C3H/s, C57BL/6J, and Balb/c). Female mice (10 to 12 wks old, mean = 22.9 g, SD = 3.2 g) were administered with nine hourly injections of 214.19 mg/kg 5-Bromo-2′ deoxyuridine (BrdU) followed by 0, 0.048, 0.449, 4.585 or 46.93 mg/kg CP and 4 mg/kg colcemid. SCEs were evaluated following differential staining procedures of Perry and Wolff (1974). The base-line SCEs were similar in all strains with about ten SCEs/cell. Increasing CP concentrations yielded an increased level of SCEs. Most cells showed extensive damage in CP doses exceeding 4.55 mg/kg. No SCE evaluation was possible beyond this concentration. Strain differences were evident at every dose of CP, and Balb/c was the least susceptible strain to SCE induction. F1 hybrids involving C3H/s ♂ and Balb/c ♀ showed SCE values closer to Balb/c. Data on the association between chromosome length and frequency of SCEs are provided. They empirically establish a positive correlation (r = 0.90) between the two features. Most induced SCEs were interstitially located rather than terminally positioned on the chromosome.This publication has 18 references indexed in Scilit:
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