Fertilization and Mouse Embryo Development in the Presence of Midazolam

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Abstract
Development were evaluated in the presence of midazolam. Midazolam was cocultured with two-cell mouse preimplantation embryos over 72 h and injected systemi-cally just before ovulation and coitus. Concentrations to 12.5 /xg/mL displayed no significant toxic effects on in vitro two-cell-to-blastocyst development. Doses to 35.0 mg/kg did not prevent or impair in vivo fertilization. Midazolam has no adverse effect on in vitro development of two-cell-to-blastocyst-stage embryos nor on in vivo fertilization and cell division at concentrations approximating and exceeding those that ova are exposed to during clinical anesthesia. Midazolam is recommended for use to induce sedation in human in vitro fertilization where association with gametes and zygotes is probable. Address correspondence to Dr. Swanson, Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529–0266. Supported in part by a grant from The Embryonics Laboratory, Old Dominion University, Norfolk, Virginia. Accepted for publication May 11, 1992. © 1992 International Anesthesia Research Society...

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