Opioid tolerance/dependence in neuroblastoma×glioma (NG108‐15) hybrid cells is associated with a reduction in spontaneous stimulatory receptor activity

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Abstract
Chronic opioid regulation of stimulatory receptor activity was investigated in neuroblastoma×glioma (NG108‐15) hybrid cells stably transfected to express the human β2‐adrenoceptor (β2‐AR). Expressed β2‐ARs are functionally coupled to G proteins and display ligand‐independent signalling activity, as demonstrated by the ability of an inverse agonist to attenuate basal adenylyl cyclase (AC) activity. Despite the relative increase in basal AC activity due to the development of tolerance/dependence, chronic morphine treatment was found to completely abolish spontaneous β2‐AR activity by reducing basal receptor/G protein precoupling. A similar chronic opioid effect was observed in transiently transfected COS‐7 cells. These results indicate that during the state of opioid tolerance/dependence basal levels of AC activity are no longer under the control of spontaneously active stimulatory receptors.