INITIATION AND CHARACTERIZATION OF CULTURED TUMOR LINES FROM SPONTANEOUS RETICULUM-CELL SARCOMA OF SJL-J MICE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (12) , 4439-4448
Abstract
Three reticulum cell sarcoma lines (LA1, LA6 and LA8) were established from SJL/J spontaneous Hodgkin''s-like reticulum cell sarcoma. All cultures are lymphoid with blast-like morphology by light microscopy and EM and produce the type B neoplasm (Dunn classification) when injected into young SJL/J mice. Identification of these tumors as lymphocytic and monocytic was investigated by surface markers, histochemical staining, and phagocytic function. La6 and LA8 bear receptors for the Fc portion of immunoglobulin complement receptors. Thy 1.2 antigens and surface immunoglobulin were not detected on any of the 3 lines. No lines were able to synthesize immunoglobulin or phagocytose Degalon beads. Histochemical staining was presumptive of lymphocytes or lymphoblasts with slight differences between the lines. Although a T[thymus-derived]- or B[bone marrow-derived]-cell classification cannot definitively be made for these tumors lines because of their lack of specific markers, the results are consistent with an immature B-cell origin for the SJL/J reticulum cell sarcoma.